GSM2572202: Treated wild-type; Deinococcus radiodurans; RNA-Seq - SRA

SRX2731649: GSM2572202: Treated wild-type; Deinococcus radiodurans; RNA-Seq
1 ILLUMINA (Illumina HiSeq 4000) run: 12.7M spots, 3.8G bases, 1.4Gb downloads

Submitted by: NCBI (GEO)

Study: RNA-seqanalysis of deinococcus wild-type and mazEF mutant strains after MMC treatment

PRJNA382319 • SRP103760 • All experiments • All runs

show Abstracthide Abstract

RNA-seq was used to analysis of mazEF toxin-antitoxin system mediated post-transcriptional inhibition through its endoribonuclease activity. DNA damage agents ,such as MMC, activates the mazEF system leading to cells growth arrest. In the present study, results reveal that a larger number of genes involved in amino acid and carbohydrate metabolism, ion transport and transcription was down-regulated in the wild-type compared with the mazEF-dr mutant. Overall design: Total RNAs were extrated from wild-type deinococcus radiodurans and mazEF mutant strain with or without MMC treatment.

Sample: Treated wild-type

SAMN06703834 • SRS2119547 • All experiments • All runs

Organism: Deinococcus radiodurans R1

Library:

Instrument: Illumina HiSeq 4000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Total RNA was extracted using the TRIzol method. Then, Dnase I was used to digest the DNA and Ribo-Zero Magnetic Kit was used to remove the rRNAs. RNA libraries were prepared for sequencing using standard Illumina protocols

Experiment attributes:

GEO Accession: GSM2572202

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 12.7M spots, 3.8G bases, 1.4Gb

Run	# of Spots	# of Bases	Size	Published
SRR5441789	12,665,957	3.8G	1.4Gb	2017-04-18

ID:: 3935861

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